Genetic Indentification of An Individual with A102Bw33 Blood Group Gene Subtypes of ABO Variant / 中国实验血液学杂志

<p><b>OBJECTIVE</b>To investigate and indentify the molecular characteristics of a sample serologically identified as ABw subgroup.</p><p><b>METHODS</b>The individual was confirmed by standard serological techniques. The genotyping and sequencing were performed using polymerase chain reaction-sequence specific primer (PCR-SSP), direct sequencing and gene cloning for exon 6 and exon 7 of ABO locus respectively.</p><p><b>RESULT</b>Both A and B antigen were detected on red blood cells of the proband and anti-B antibody was detected in his serum. PCR-SSP showed that the sample gene was A1B phemotype. DNA sequencing showed 297A/G, 467C/T, 526C/G, 657C/T, 703A/G, 803G/C and 930G/A heterozygote in exon 6 to exon 7. After cloning and sequencing, 2 alleles A102 and Bw33 were obtained. The sequence of Bw33 allele had one nucleotide change (A to C) at position 796 compared with that of B101 allele. The nucleotide in this B allele at site 796 is a "C" characteristic of the A form of the allele.</p><p><b>CONCLUSION</b>A>C at nt796 of α-1, 3 galactosyltransferase gene can result in Bw33 phenotype with the anti-B antibody in serum.</p>

Surgical treatment of thoracic tuberculosis with one stage posterior debridement and bone grafting fusion and internal fixation / 中国骨伤

<p><b>OBJECTIVE</b>To investigate the effect and indication of one stage posterior debridement and bone grafting fusion and internal fixation for thoracic tuberculosis.</p><p><b>METHODS</b>From January 2005 to May 2011,12 patients with thoracic tuberculosis were treated with one stage posterior debridement and pedicle screw fixation combined with regular anti-tuberculosis treatment before and after operation. There were 7 males and 5 females,with an average age of 45 years and average course of 15 months. Information of operative time, blood loss, bony fusion, local kyphosis and neurologic functional were evaluated.</p><p><b>RESULTS</b>All infective focus were thoroughly removed and bone graft obtained fusion. The mean of operative time and blood loss were 170 min (120-210 min) and 510 ml (200-1 000 ml),respectively. Cobb angle from (28.7 +/- 9.2) degrees preoperatively decreased to (8.2 +/- 3.5) degrees postoperatively(P<0.05). No kyphosis correction loss,tubercular recurrence or failure of internal fixation was found. According to Frankel grade to evaluate neurological function, all patients arrived to grade E.</p><p><b>CONCLUSION</b>One stage posterior debridement and bone grafting fusion and internal fixation is an effective method in treating thoracic tuberculosis. It has advantages such as thorough debridement, short operative time, less blood loss, more kyphosis correction and higher bony fusion rate.</p>

Expression of neutrophil adhesion molecule CD11b as an early diagnostic marker for neonatal sepsis / 中华儿科杂志

<p><b>OBJECTIVE</b>Neonatal sepsis is a common disease and the sepsis-related mortality rate is still high. Until now, there has no ideal diagnostic marker to early identify neonatal sepsis. Expression of neutrophil adhesion molecule CD(11b) was showed as the earlier reaction to the infection/inflammation, and may be applied as an early diagnostic marker for sepsis. This study was to investigate this antigen for early diagnosis of neonatal sepsis related to bacterial infection.</p><p><b>METHODS</b>According to clinical symptoms, signs and four indices (WBC, PLT, plasma CRP and ratio of I/T), fifty-one neonates with established or suspected sepsis were allocated retrospectively into two groups of sepsis [n = 23, gestational age of (38.3 +/- 2.4) weeks, postnatal age of (12.7 +/- 8.8) days, body weight: (3.1 +/- 0.8) kg] and suspected sepsis [n = 28, gestational age of (38.8 +/- 1.6) weeks, postnatal age of (11.7 +/- 7.3) days, body weight: (3.3 +/- 0.6) kg]. Fifteen healthy neonates were served as controls [gestational age: (38.5 +/- 1.4) weeks, postnatal age: (8.2 +/- 5.5) days, body weight: (3.3 +/- 0.3) kg]. CD(11b) was quantified with the whole blood flow cytometry and direct immunofluorescence technique.</p><p><b>RESULTS</b>The expressions of neutrophil CD(11b) in neonates with sepsis and suspected sepsis were (320 +/- 189) MFI and (456 +/- 213) MFI, respectively, which was lower than that of controls [(1,090 +/- 338) MFI, t = -9.01 and -7.56, respectively; P < 0.001]. The expression of CD(11b) was lower in neonates with sepsis than that with suspected sepsis (t = -2.39, P < 0.05). The expression of CD(11b) in neonates with CRP >or= 30 mg/L was (211 +/- 164) MFI, which was lower than those with CRP < 30 mg/L [(505 +/- 265) MFI, t = 2.64, P < 0.05]. The detection of CD(11b) (<or= 600 MFI) for suspected sepsis showed a sensitivity of 86.3%, a specificity of 100%, a positive predictive value of 100%, and a negative predictive value of 68.2%. The positive rate of CD(11b) detection was 86.3%, which was higher than the blood culture test (17.6%, chi(m)(2) = 31.2, P < 0.05).</p><p><b>CONCLUSION</b>The expression of CD(11b) in neonatal sepsis presented with a down-regulation and, the decreased CD(11b) expression might be related to the severity of infections. For the neonatal sepsis the serial measurements of neutrophil CD(11b) expression with the whole blood flow cytometry seemed feasible and reliable in the early diagnosis, evaluation of infection severity and observation of therapy reactions.</p>